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UHPLC/MS Analysis of Ten Cannabinoids on Ascentis® Express RP-Amide

UHPLC/MS Analysis of Ten Cannabinoids on Ascentis® Express RP-Amide application for UHPLC-MS

Materials

related product

Product No.
Description
Pricing

53913-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

C-045

Cannabidiol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-046

Cannabinol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-005

(−)-trans-Δ9-THC solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-140

Cannabidivarin solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-141

Cannabigerol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-142

Cannabigerolic acid solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

C-143

Cannabichromene solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-144

Cannabidiolic acid solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

T-094

Tetrahydrocannabivarin (THCV) solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-093

Delta9-Tetrahydrocannabinolic acid A solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.0 μm particles (53913-U)

mobile phase

[A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile

gradient

60% B to 100% B in 10 min

flow rate

0.4 mL/min

pressure

7600 psi (524 bar)

column temp.

35 °C

detector

MS, ESI(+), ESI(-), MRM

injection

1 μL

sample

1000 ng/mL each in methanol

Description

Analysis Note

Cannabis compounds reportedly have therapeutic efficacy in the treatment of pain, mood disorders, and inflammatory diseases. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing. Shown here is the separation of cannabis compounds on an Ascentis Express RP-Amide column. Highest grade UHPLC solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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