UHPLC Analysis of Conjugated Estrogens on Titan C18
Materials
related product
Product No.
Description
Pricing
CONDITIONS
column
Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)
mobile phase
[A] 10 mM potassium phosphate, pH 7.0; [B] acetonitrile; [C] methanol
gradient
90:5:5 (A:B:C) to 75:20:5 (A:B:C) in 4.35 min; to 40:45:15 (A:B:C) in 6.75 min; to 90:5:5 (A:B:C) in 7 min
flow rate
0.6 mL/min
pressure
9500 psi (655 bar)
column temp.
35 °C
detector
UV, 220 nm
injection
2 μL
sample
50 μg/mL each in water:acetonitrile (60:40 )
Description
Analysis Note
The Titan C18 column provided efficient and rapid resolution of thirteen related estrogenic compounds. Ultra Ultra high purity solvents provided robust operation.
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