推荐产品
生物来源
bacteriophage MS2
质量水平
形式
solution
分子量
~1200 kDa
包装
pkg of 500 μL (10 A260 units)
制造商/商品名称
Roche
技术
RNA extraction: suitable
颜色
colorless
溶解性
water: miscible
储存温度
−20°C (−15°C to −25°C)
一般描述
MS2噬菌体RNA由3569个核苷酸组成。MS2噬菌体在分子生物学研究中是模式系统，可用于病毒RNA复制，感染细胞翻译和生理学研究。MS2 RNA编码三个病毒多肽链，即蛋白质A、外壳蛋白和RNA复制酶复合物。蛋白质A和外壳蛋白组成了MS2病毒的结构。
应用
RNA，MS2用于：
- 评估RNA定量方法的标准品
- RNA提取
- 单管荧光PERT检测（STF-PERT），对不同逆转录病毒类型进行准确定量
- 各种研究，在体内或体外使用天然RNA的蛋白合成系统，起始、延长或终止多肽链合成。
- 结构和功能研究。
- 在cDNA合成过程中稳定RNA
- 在RNA提取或分离过程中提高RNA产量
质量
典型分析：根据现在的质量控制步骤，不会有蛋白质和宿主核酸。
序列
链长度3569个核苷酸
制备说明
工作溶液：Storage buffer: 10 mM Tris-HCl，1 mM EDTA，pH 7.0。
其他说明
仅用于生命科学研究。不可用于诊断。
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
No data available
闪点(°C)
No data available
法规信息
常规特殊物品
Roman Aranda et al.
Analytical biochemistry, 387(1), 122-127 (2009-05-21)
Quantification of RNA is essential for various molecular biology studies. In this work, three quantification methods were evaluated: ultraviolet (UV) absorbance, microcapillary electrophoresis (MCE), and fluorescence-based quantification. Viral, bacterial, and eukaryotic RNA were measured in the 500 to 0.05-ng microl(-1)
Petra Vychytilova-Faltejskova et al.
Carcinogenesis, 37(10), 941-950 (2016-08-04)
Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of this study was to identify serum microRNAs enabling early
Aitana Braza-Boïls et al.
International journal of molecular sciences, 21(8) (2020-04-25)
There is evidence for the effects of platelet inhibition on innate immune activation. Circulating microRNAs (miRNAs) have been implicated as markers of platelet and leukocyte activation. In the present study, we assessed the effects of P2Y12 inhibitors on platelet and
Thomas C Roberts et al.
Biological procedures online, 16(1), 5-5 (2014-03-19)
MicroRNAs (miRNAs) are short RNA molecules which regulate gene expression in eukaryotic cells, and are abundant and stable in biofluids such as blood serum and plasma. As such, there has been heightened interest in the utility of extracellular miRNAs as
Niina Vuokila et al.
Neuroscience, 433, 21-35 (2020-03-07)
Mechanisms initiated by traumatic brain injury (TBI), leading to the development of progressive secondary injury are poorly understood. MicroRNAs (miRNAs) have a proposed role in orchestrating the post-injury aftermath as a single miRNA can control the expression of several genes.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门