Merck
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CAPHOS

Sigma-Aldrich

磷酸钙转染试剂盒

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

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Synonym(s):
Gene delivery
EC 号:
233-140-8
NACRES:
NA.85

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等级

for molecular biology

质量水平

200

形式

solution

用途

1 kit sufficient for 160 transfections (6 cm dishes)
1 kit sufficient for 400 transfections (3.5 cm dishes)
1 kit sufficient for 80 transfections (10 cm dishes)

technique(s)

transfection: suitable

运输

dry ice

储存温度

−20°C

Related Categories

一般描述

磷酸钙法转染是将DNA引入真核细胞的常用方法。这一技术已经在广泛的细胞类型中使用，可实现瞬时和稳定的转染效果。

应用

磷酸钙转染试剂盒用于：
  • 启动转染
  • 转染 Hek293T 细胞
  • 转染 H29D 细胞

适用于瞬时和稳定地将DNA转染至培养的哺乳动物细胞中。 使用磷酸钙方法已成功转染了以下细胞：

BAEC
肠黑素瘤细胞
CHO K1
COS-7
成纤维细胞（人胚胎、新皮肤）
HEK293
Huh7
IMR-90
LLC（Lewis肺癌）
NIH3T3
PC-12
PCI-13
SH-SY5Y
SK-HEP-1
T47D

包装

1 kit

特点和优势

  • 适用于瞬时和稳定转染
  • 可重复用于多种细胞类型
  • 广泛引用
  • 价格便宜

组分

磷酸钙转染试剂盒包含：
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES 缓冲盐 (H1012)
25 ml 分子生物学级纯水(W4502)

原理

该方法是基于将含有磷酸钠的HEPES缓冲盐水和含有DNA的CaCl2 溶液进行缓慢混合实现的。 所形成的DNA-磷酸钙共沉淀物可粘附于细胞表面，并可能通过胞吞作用被细胞吸收。

其他说明

该方案可用于使用pSV40-CAT质粒作为报告基因对CHO细胞进行瞬时转染。

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H319

危险分类

Eye Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Product Information Sheet - CAPHOS

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