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E6908

Sigma-Aldrich

pFLAG-CMV™-5.1 Expression Vector

shuttle vector for intracellular transient expression of C-terminal FLAG

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About This Item

UNSPSC代码:
12352200

标签

FLAG® tagged

等级

Molecular Biology

表单

buffered aqueous solution

肽标签位置

C-terminal

运输

dry ice

储存温度

−20°C

一般描述

The pFLAG-CMV™-5.1 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector, used for establishing transient intracellular expression of C-terminal FLAG® fusion proteins in mammalian cells.

pFLAG-CMV-5.1 is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.

The pFLAG-CMV-5b-BAP Control Plasmid is a 6.0 kb derivative of the pCMV5 transient expression vector for intracellular expression of C-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

应用

pFLAG-CMV™-5.1 Expression Vector is suitable for transient or stable expression and secretion of N-terminal FLAG®-tagged fusion proteins in mammalian cells.

组分

  • pFLAG-CMV™-5.1 Expression Vector 20 μg (E7901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • pFLAG-CMV™-5b-BAP Control Plasmid 20 μg (P5225) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

原理

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs. The multiple cloning region of the pFLAG-CMV™-5.1 vector is not preceded by a translational initiation signal. A translational start sequence must be included with the insert DNA. The multiple cloning region of the pFLAG-CMV-5.1 vector is compatible with other CMV vectors.

法律信息

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

10 - Combustible liquids

历史批次信息供参考:

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