p3xFLAG-Myc-CMV^™-25 Expression Vector

The p3XFLAG-Myc-CMV^™-25 Expression Vector is a 6.4 kb derivative of pCMV5 used to establish transient or stable expression of secreted, dual tagged N-terminal 3XFLAG^™and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. The incorporation of 3XFLAG in the expression vector results in increased detection sensitivity using ANTI-FLAG M2 antibody.

p3XFLAG-Myc-CMV-25 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.

The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.

Vector Maps and Sequences

p3XFLAG-Myc-CMV^™-25 Expression Vector 20 μg (E6276) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
p3XFLAG-CMV^™-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG^® and c-myc fusion constructs. The aminoglycoside phosphotransferase II gene (Neo-r) confers resistance to aminoglycosides such as G418 allowing for selection of stable transfectants.

This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.

3xFLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

p3xFLAG-Myc-CMV is a trademark of Sigma-Aldrich Co. LLC