MSSAFE
Protease and Phosphatase Inhibitor Cocktail
MS-SAFE, powder, Protease inhibitors: serine, cysteine and aspartic proteases and metalloproteases. Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases.
别名:
质谱安全蛋白酶和磷酸酶抑制剂混合物
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About This Item
UNSPSC代码:
12352200
NACRES:
NA.77
表单:
powder
溶解性:
water: 0.1 g/mL, clear, colorless
储存温度:
2-8°C
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一般描述
质谱(MS)兼容蛋白酶抑制剂鸡尾酒和磷酸酶抑制剂鸡尾酒,具有广泛的抑制特异性:
- 丝氨酸、半胱氨酸和天冬氨酸蛋白酶以及金属蛋白酶
- 酪氨酸、丝氨酸/苏氨酸、酸性和碱性磷酸酶
特异性
蛋白酶抑制剂:丝氨酸、半胱氨酸和天冬氨酸蛋白酶以及金属蛋白酶。
磷酸酶抑制剂:酪氨酸、丝氨酸/苏氨酸、酸性和碱性磷酸酶
磷酸酶抑制剂:酪氨酸、丝氨酸/苏氨酸、酸性和碱性磷酸酶
应用
MS-SAFE蛋白酶和磷酸酶抑制剂已用于测量核糖体蛋白S6激酶(S6K)活性。
在哺乳动物细胞裂解物和肝组织提取物中测试。设计用于质谱分析的样本。
特点和优势
用于质谱分析的综合蛋白酶和磷酸酶抑制剂混合物
与下游样品处理兼容,如 His 标记的蛋白纯化和磷酸肽富集
允许精确测量蛋白活性和鉴定磷酸化位点。
与下游样品处理兼容,如 His 标记的蛋白纯化和磷酸肽富集
允许精确测量蛋白活性和鉴定磷酸化位点。
组分
蛋白酶抑制剂:
- 贝他定盐酸
- 亮抑酶肽
- 磷酰胺基二钠盐
- 胃蛋白酶抑制剂
- 弹性酶抑制剂
- 抑肽酶
- 甲磺酸萘福莫司他
- 抗蛋白酶
- 豆渣酸
- 氟化钠
- 原钒酸钠
- 草酸溴四胺酯
- β-乳糖
- DL-亮氨酸
数量
一小瓶使用水或提取/裂解缓冲液制备20 mL 1倍抑制剂鸡尾酒工作溶液。或者,可以通过加入2 mL水或提取/裂解缓冲液来制备10倍的浓缩液。然后,根据1倍工作溶液的需要,可以将该10倍溶液10倍稀释到提取/裂解缓冲液中。
外形
水溶性冻干粉末
警示用语:
Warning
危险声明
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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Mark D Schuchard et al.
BioTechniques, 39(2), 239-247 (2005-08-25)
The inclusion of protease inhibitors in serum or plasma samples has been found to significantly impact the isoform profile of selected plasma proteins as seen on 2-dimensional electrophoresis (2-DE) gels. With the addition of a protease inhibitor cocktail, several human
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相关内容
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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