pT7-FLAG^™-1 Expression Vector

pT7-FLAG^™-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a N-terminal Met-FLAG fusion protein containing the FLAG^® epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

pT7-FLAG^®-1 Expression Vector has been used in the cloning of ubiquitin fusion protein and connexin 43 (Cx43) C-terminal fragment.

pT7-FLAG^™-1 Expression Vector (P6742) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pT7-FLAG^™-1-BAP Control Vector (P6992) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC