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Merck
CN

901757

Sigma-Aldrich

Low endotoxin gelatin from porcine skin

gel strength 100 (Bloom), <10 EU/g

别名:

beMatrix™ gelatin LS-W

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关于此项目

化学文摘社编号:
9000-70-8
UNSPSC代码:
41116130
NACRES:
NA.23

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表单

powder

杂质

<10 EU/g Endotoxin
<10 EU/g
<300 g Total viable aerobic count

缺失

<15% loss on drying

颜色

white to pale yellow

pH值（酸碱度）

5.0-6.5

粘度

1.8-2.8 mPa.s

凝胶强度

80-120 (bloom)

痕量阴离子

sulfite (SO32-): <1.5 mg/ 20 g

痕量阳离子

As: <1 ppm
Hg: <0.1 ppm
heavy metals: <20 ppm

储存温度

2-8°C

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应用

Gelatin has received significant attention in the biomedical field due to its inherent bioactivity. Low endotoxin gelatin from porcine skin, gel strength 100 bloom is a low bloom (low molecular weight) gelatin featuring less than 10 endotoxin units (EU) per gram. Endotoxins primarily consist of lipopolysaccharides from the outer membrane of gram-negative bacteria. Once in the bloodstream, endotoxins are capable of eliciting a strong immune response, potentially leading to fever, shock, a drop in blood pressure, and even potentially death. In addition to this immune response, several reports suggest endotoxins may also damage various types of cells. To avoid adverse effects, raw materials should contain the lowest level of endotoxins possible when used in biomedical or regenerative medicine research applications. This low endotoxin gelatin is derived from alkaline-treated porcine skin and has a variety of potential applications in the fields of regenerative medicine, medical devices, and tissue engineering research.

法律信息

beMatrix is a trademark of Nitta Gelatin

储存分类代码

11 - Combustible Solids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCP6230
MKCH0151

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T Kirikae et al.
International journal of immunopharmacology, 19(5), 255-262 (1997-05-01)
Trace amounts of endotoxin (lipopolysaccharide: LPS) are assumed to contaminate commercially available fetal bovine serum (FBS) for tissue or cell culture during the manufacturing process. We examined how cultured cells were affected by the endotoxin and how much endotoxin was
K W Brunson et al.
Journal of supramolecular structure, 9(2), 231-242 (1978-01-01)
Chinese hamster ovary (CHO . K1 . PRO) cell growth was inhibited by addition of a gram-negative bacterial lipopolysaccharide (LPS) to the cell culture medium. Growth inhibition began after three or four days of incubation, was dose-dependent up to a
J Ongrádi et al.
Folia microbiologica, 29(6), 450-454 (1984-01-01)
Cytotoxicity of a mixed pyrogen preparation and its components as well as native and radiodetoxified lipopolysacharides (LPS) was determined with established HEp-2 cell cultures and by measuring plating efficiency. This proved to be more sensitive to the damaging effect of
E J Ziegler et al.
The New England journal of medicine, 307(20), 1225-1230 (1982-11-11)
In an effort to decrease deaths from gram-negative bacteremia and endotoxin shock, we treated bacteremic patients with human antiserum to endotoxin (lipopolysaccharide) core. Antiserum was prepared by vaccinating healthy men with heat-killed Escherichia coli J5; this mutant lacks lipopolysaccharide oligosaccharide
J G Brock-Utne et al.
Anaesthesia and intensive care, 17(1), 49-55 (1989-02-01)
Endotoxins (lipopolysaccharides, LPS) are potent bacterial poisons always present within the intestines in considerable amounts. Several pathophysiological conditions such as hypovolaemia, hypoxia, intestinal ischaemia, burns and radiation lead to a breakdown in the barrier and depending upon the extent of
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