产品名称
16:0-14 Doxyl PC, Avanti Research™ - A Croda Brand 810605P, powder
assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 1 mg (810605P-1mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand 810605P
shipped in
dry ice
storage temp.
−20°C
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General description
16:0-14 Doxyl Phosphatidylcholine (PC) is a doxyl probe with a nitroxyl ring attached at carbon 14 on the chain of 1-palmitoyl-2-stearoyl-(14-doxyl)-sn-glycero-3-phosphocholine (PSPC).
Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.
Application
16:0-14 Doxyl PC (1-palmitoyl-2-stearoyl-(14-doxyl)-sn-glycero-3-phosphocholine) has been used:
- in the labeling of small unilamellar vesicles for electron spin resonance (ESR) spectra
- as spin label for labeling at different depths of 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (PSPC) multilamellar vesicles (MLVs) for nuclear magnetic resonance (NMR) studies
- as paramagnetic spin labels for 1H-15N heteronuclear single quantum coherence (HSQC) spectra
Biochem/physiol Actions
16:0-14 Doxyl Phosphatidylcholine (PC) is used as a label to probe various membrane depths from the hydrocarbon-water interface. It quenches the fluorescence of 7-nitrobenz-2-oxa-1,3-diazole (NBD), a fluorescent label in the lipid bilayer. There is a progressive increase in the depth and degree of mobility of the probe as the doxyl group substitution proceeds towards the end of the lipid chain, and flexibility also increases.
Packaging
5 mL Clear Glass Sealed Ampule (810605P-1mg)
Preparation Note
Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
存储类别
11 - Combustible Solids
法规信息
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