SMC^® 2-Plex Human IL-17A, IL-17F High Sensitivity Immunoassay Kit

The IL-17 cytokine family is vital for protective immunity and inflammatory pathology during infection and autoimmunity. Dysregulated IL-17 responses can lead to immunopathology in various disorders, making the IL-17 pathway a key drug target in autoimmune and chronic inflammatory disorders. Understanding the multifunctional properties of IL-17 is crucial for future studies aiming to regulate its expression and function, particularly in the context of autoimmune diseases and tumors.

InflammatoryDisease / Immune Response / Autoimmune Disease

96well assay

IL-17A:

• Sensitivity: LLOQ 20 fg/mL* (FemtoQuest™ Instrument)
• Standard Curve Range: 20-60,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Plasma 82-186%, Serum 61-106%
• Recovery: Plasma 94-121%, Serum 88-112%

IL-17F

• Sensitivity: LLOQ 90 fg/mL* (FemtoQuest™ Instrument)
• Standard Curve Range: 90-300,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Plasma 31-108%, Serum 77-117%
• Recovery: Plasma 79-107%, Serum 79-101%

*Indicative LLOQ created from average of 14 independent in house runs, where ≥ 70% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

AlthoughIL-17A was detectable in non-human primate, mouse, rat, canine, and felinesamples, the assay has not been verified for these species.

AlthoughIL-17F was detectable in non-human primate, rat, and feline samples, the assayhas not been verified for these species.

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

Ourproducts are intended for research use only and are not to be used for anyother purpose, which includes but is not limited to, unauthorized commercialuses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any typeof consumption or application to humans or animals.