生物来源
mouse
质量水平
抗体形式
purified antibody
克隆
56, monoclonal
纯化方式
using protein G
种属反应性
rat, human, mouse
制造商/商品名称
Upstate®
技术
ChIP: suitable
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
一般描述
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression of several yeast genes.
~25 kDa
免疫原
Epitope: Ubiquityl Lys120
Immunogen used was a branched peptide corresponding to the ubiquitination site on human H2B.
应用
Anti-Ubiquityl-Histone H2B Antibody, clone 56 is a Mouse Monoclonal Antibody for detection of Ubiquityl-Histone H2B also known as H2BUb, Histone H2B (ubiquityl), HIST1H2B & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
生化/生理作用
Based on sequence homology, broad species cross-reactivity is expected.
Recognizes histone H2B when it is ubiquitinated at Lys120 but not H2B in its unmodified state, or ubiquitin unconjugated to H2B. Mr ~25 kDa.
外形
Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
Format: Purified
Format: Purified
制备说明
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
分析说明
Control
HeLa cell lysate.
HeLa cell lysate.
Routinely evaluated by Western Blot
Western Blot Analysis:
A 0.25 μg/mL dilution of a recent lot detected ubiquityl histone H2B in HeLa cells.
Western Blot Analysis:
A 0.25 μg/mL dilution of a recent lot detected ubiquityl histone H2B in HeLa cells.
其他说明
Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
新产品
此项目有
Germano Cecere et al.
Molecular cell, 50(6), 894-907 (2013-06-29)
The inhibition of transcriptional elongation plays an important role in gene regulation in metazoans, including C. elegans. Here, we combine genomic and biochemical approaches to dissect a role of ZFP-1, the C. elegans AF10 homolog, in transcriptional control. We show that ZFP-1
Anna Mosbech et al.
The Journal of biological chemistry, 288(23), 16579-16587 (2013-04-26)
Protein recruitment to DNA double-strand breaks (DSBs) relies on ubiquitylation of the surrounding chromatin by the RING finger ubiquitin ligases RNF8 and RNF168. Flux through this pathway is opposed by several deubiquitylating enzymes (DUBs), including OTUB1 and USP3. By analyzing
Lipeng Wu et al.
Molecular cell, 49(6), 1108-1120 (2013-03-05)
Crosstalk between H2B ubiquitylation (H2Bub) and H3 K4 methylation plays important roles in coordinating functions of diverse cofactors during transcription activation. The underlying mechanism for this trans-tail signaling pathway is poorly defined in higher eukaryotes. Here, we show the following:
Jiali Jin et al.
Nature communications, 7, 13594-13594 (2016-11-26)
Nanog is a master pluripotency factor of embryonic stem cells (ESCs). Stable expression of Nanog is essential to maintain the stemness of ESCs. However, Nanog is a short-lived protein and quickly degraded by the ubiquitin-dependent proteasome system. Here we report
Roman Kessler et al.
Nature communications, 6, 7049-7049 (2015-04-29)
dDsk2 is a conserved extraproteasomal ubiquitin receptor that targets ubiquitylated proteins for degradation. Here we report that dDsk2 plays a nonproteolytic function in transcription regulation. dDsk2 interacts with the dHP1c complex, localizes at promoters of developmental genes and is required
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