biological source
mouse
Quality Level
antibody form
purified antibody
clone
56, monoclonal
purified by
using protein G
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression of several yeast genes.
~25 kDa
Immunogen
Epitope: Ubiquityl Lys120
Immunogen used was a branched peptide corresponding to the ubiquitination site on human H2B.
Application
Anti-Ubiquityl-Histone H2B Antibody, clone 56 is a Mouse Monoclonal Antibody for detection of Ubiquityl-Histone H2B also known as H2BUb, Histone H2B (ubiquityl), HIST1H2B & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Based on sequence homology, broad species cross-reactivity is expected.
Recognizes histone H2B when it is ubiquitinated at Lys120 but not H2B in its unmodified state, or ubiquitin unconjugated to H2B. Mr ~25 kDa.
Physical form
Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
Format: Purified
Format: Purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Control
HeLa cell lysate.
HeLa cell lysate.
Routinely evaluated by Western Blot
Western Blot Analysis:
A 0.25 μg/mL dilution of a recent lot detected ubiquityl histone H2B in HeLa cells.
Western Blot Analysis:
A 0.25 μg/mL dilution of a recent lot detected ubiquityl histone H2B in HeLa cells.
Other Notes
Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
存储类别
10-13 - German Storage Class 10 to 13
法规信息
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Germano Cecere et al.
Molecular cell, 50(6), 894-907 (2013-06-29)
The inhibition of transcriptional elongation plays an important role in gene regulation in metazoans, including C. elegans. Here, we combine genomic and biochemical approaches to dissect a role of ZFP-1, the C. elegans AF10 homolog, in transcriptional control. We show that ZFP-1
Vikki M Weake et al.
Genes & development, 25(14), 1499-1509 (2011-07-19)
The Spt-Ada-Gcn5-acetyltransferase (SAGA) complex was discovered from Saccharomyces cerevisiae and has been well characterized as an important transcriptional coactivator that interacts both with sequence-specific transcription factors and the TATA-binding protein TBP. SAGA contains a histone acetyltransferase and a ubiquitin protease.
Manjeet Kaur Gill-Sharma et al.
Clinical epigenetics, 4(1), 23-23 (2012-12-18)
The putative regulatory role of the male reproductive hormones in the molecular mechanism underlying chromatin condensation remains poorly understood. In the past decade, we developed two adult male rat models wherein functional deficits of testosterone or FSH, produced after treatments