05-185
Anti-avian Src Antibody, clone EC10
clone EC10, Upstate®, from mouse
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
EC10, monoclonal
Application:
ICC, IP, WB
Citations:
45
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
EC10, monoclonal
species reactivity
avian
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SRC(6714)
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General description
60 kDa
Immunogen
Recombinant chicken pp60src
Application
Anti-avian Src Antibody, clone EC10 detects level of avian Src & has been published & validated for use in IP, WB & IC.
Biochem/physiol Actions
specific only for avian Src; this antibody is excellent for distinguishing pp60src in cells transfected with v-src or c-src expression vectors.
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, with 0.05% sodium azide
Format: Purified
Analysis Note
routinely evaluated in (60kDa) on a RIPA lysate of chicken embryo fibroblasts (CEF)
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Normal cells control the growth of neighboring transformed cells independent of gap junctional communication and SRC activity.
David B Alexander, Hitoshi Ichikawa, John F Bechberger, Virginijus Valiunas, Misao Ohki et al.
Cancer Research null
Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene.
Reynolds, A B, et al.
Molecular and cellular biology, 9, 629-638 (1989)
Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin
Parsons, S. J., et al
Journal of virology, 51, 272-282 (1984)
Wanping Xu et al.
Molecular cell, 47(3), 434-443 (2012-06-26)
Many critical protein kinases rely on the Hsp90 chaperone machinery for stability and function. After initially forming a ternary complex with kinase client and the cochaperone p50(Cdc37), Hsp90 proceeds through a cycle of conformational changes facilitated by ATP binding and
Tulsiram Prathapam et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(8), 2695-2700 (2006-02-16)
The transcription factor Myc plays a central role in the control of cellular proliferation. Myc expression is induced by growth factors in a pathway mediated by cellular Src (c-Src), but it is not clear whether Myc induction or activity is
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