05-414
Anti-CrkL Antibody, clone 5-6
clone 5-6, Upstate®, from mouse
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5-6, monoclonal
Application:
immunohistochemistry
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Species reactivity:
mouse, rat, human
Citations:
15
Technique(s):
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-CrkL Antibody, clone 5-6, clone 5-6, Upstate®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
5-6, monoclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CRKL(1399)
Analysis Note
Control
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells
Routinely evaluated by Western Blot on K562 cell lysate.
Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
Application
Detect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Other species have not been tested.
the C-terminal SH3 of Human CRKL
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
39kDa
CRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
The antibody does not cross-react with CRKI or CRKII.
The antibody does not cross-react with CRKI or CRKII.
Immunogen
Epitope: Full-length
Full length GST fusion protein corresponding to Human CRKL
Physical form
Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Atsushi Oda et al.
The Journal of biological chemistry, 278(8), 6456-6460 (2003-01-11)
Searching for proteins in platelets that can interact with the N-terminal SH3 domain of CrkL (using a combination of a pull-down assay followed by mass spectrometry), we have found that human platelets express an ADP-ribosylation factor (Arf)-specific GTPase-activating protein (GAP)
The proto-oncogene product p120CBL and the adaptor proteins CRKL and c-CRK link c-ABL, p190BCR/ABL and p210BCR/ABL to the phosphatidylinositol-3' kinase pathway
Sattler, M, et al
Oncogene, 12, 839-846 (1996)
Cellular interactions of CRKL, and SH2-SH3 adaptor protein
ten Hoeve, J, et al
Cancer Research, 54, 2563-2567 (1994)
Eike R Hrincius et al.
Cellular microbiology, 12(6), 831-843 (2010-01-22)
The non-structural protein 1 (A/NS1) of influenza A viruses (IAV) harbours several src-homology domain (SH) binding motifs that are required for interaction with cellular proteins. The SH3 binding motif at aa212-217 [PPLPPK] of A/NS1 was shown to be essential for
Tomomi Kanazawa et al.
The FEBS journal, 288(19), 5613-5628 (2021-03-27)
Adapter proteins CRK and CRKL participate in a variety of signaling pathways, including cell adhesion, and fate regulation of mammalian cells. However, the molecular functions of CRK/CRKL in epigenetic regulation remain largely unknown. Here, we developed a pipeline to evaluate
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