05-484
Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3
clone 20G3, Upstate®, from mouse
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
20G3, monoclonal
Species reactivity:
human, mouse
Application:
IP, WB
Citations:
7
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
20G3, monoclonal
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (non-phospho-Tyr1173)
Quality Level
Gene Information
human ... EGFR(1956)
mouse ... Egfr(13649)
General description
175kDa
Immunogen
peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor
Application
Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.
Physical form
100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Format: Purified
Thiophilic adsoption and size exclusion chromatography
Preparation Note
2 years at -20°C
Analysis Note
routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells
Other Notes
Replaces: 04-337; 04-341
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 1
Gang Chen et al.
Biological procedures online, 13, 1-1 (2011-03-04)
Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was estimated by RT-qPCR
Alteration of epidermal growth factor receptor activity by mutation of its primary carboxyl-terminal site of tyrosine self-phosphorylation.
Bertics, P J, et al.
The Journal of Biological Chemistry, 263, 3610-3617 (1988)
Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors.
Sorkin, A, et al.
The Journal of Biological Chemistry, 267, 8672-8678 (1992)
Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice.
Siggs, OM; Grieve, A; Xu, H; Bambrough, P; Christova, Y; Freeman, M
Biology Open null
Narendra Padhan et al.
BMC cancer, 16(1), 683-683 (2016-08-27)
The progression of colorectal cancer (CRC) involves recurrent amplifications/mutations in the epidermal growth factor receptor (EGFR) and downstream signal transducers of the Ras pathway, KRAS and BRAF. Whether genetic events predicted to result in increased and constitutive signaling indeed lead
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