生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
culture supernatant
抗体产品类型
primary antibodies
克隆
3G4B2, monoclonal
种属反应性
human, canine, monkey
制造商/商品名称
Upstate®
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... NCL(4691)
一般描述
100kDa
Nucleolin, also known as C23, is the major nucleolar protein of exponentially growing eukaryotic cells. It is a ubiquitous conserved multi-domain phosphoprotein found in all vertebrate species. Nucleolin contains a specific bipartite nuclear localization signal sequence and three different structural domains. Nucleolin is a multifunctional protein involved in most steps of ribosome biogenesis. Nucleolin has been implicated in chromatin structure, rDNA transcription, rRNA maturation, ribosome assembly, cytokinesis, nucleogenesis, cell proliferation and growth, and
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.
免疫原
Nuclear matrix extract from MDCK cells
应用
Anti-Nucleolin Antibody, clone 3G4B2 is a high quality Mouse Monoclonal Antibody for the detection of Nucleolin & has been validated in IP, WB, ICC.
生化/生理作用
nucleolin
外形
mouse culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%
分析说明
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
routinely evaluated by immunoblot on HeLa nuclear extract
其他说明
Replaces: MABE466
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
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