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抗磷酸组蛋白H2A.X（Ser139）抗体，克隆JBW301

clone JBW301, Upstate®, from mouse

别名:

H2AXS139P, Histone H2A.X (phospho S139)

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25 μG
CN¥1,527.90
200 μG
CN¥9,037.64

CN¥1,527.90

预计发货时间July 28, 2025详情

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25 μG
CN¥1,527.90
200 μG
CN¥9,037.64

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥1,527.90

预计发货时间July 28, 2025详情

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生物来源

mouse

质量水平

100

抗体形式

affinity purified immunoglobulin

克隆

JBW301, monoclonal

种属反应性

vertebrates

包装

antibody small pack of 25 μg

制造商/商品名称

Upstate®

技术

ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG1

NCBI登记号

NP_002096

相关类别

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此商品
05636I16202A16193
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 clone JBW301, Upstate®, from mouse

05636

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 clone JBW301, from mouse

05636I

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate clone JBW301, Upstate®, from mouse

16202A

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate clone JBW301, Upstate®, from mouse

16193

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

species reactivity

vertebrates

species reactivity

human, rat, mouse

species reactivity

human

species reactivity

human

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

JBW301, monoclonal

clone

JBW301, monoclonal

clone

JBW301, monoclonal

clone

JBW301, monoclonal

technique(s)

ChIP: suitable, immunofluorescence: suitable, western blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

ChIP: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

一般描述

组蛋白H2A是参与真核细胞染色质结构的5种主要组蛋白之一。H2A具有一个主要的球状结构域和一个长N末端的尾巴，其与核糖体核小体′串珠′结构体的结构有关。
17kda

特异性

抗磷酸组蛋白H2A X（Ser139）抗体（克隆JBW301）可以识别在Ser139处磷酸化的组蛋白H2A。

免疫原

对应于人组蛋白H2A.X氨基酸134-142的多肽（C-KATQA [pS] QEY）

应用

其他参考应用：
免疫组织化学分析：一个代表性批次产品在RNF168-WT和RNF 168-SA/SEKI小鼠肺组织切片（石蜡）中检测到组蛋白H2A.X（pSer139）。(Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331)。
染色质免疫沉淀，请在“技术信息”选项卡中参见Meier, Andreas, et al. EMBO J., 26: 2707-18（2007）。
抗磷酸组蛋白H2A.X（Ser139），克隆JBW301，是一种已被大量报道的小鼠单克隆抗体，已在ChIP、ICC、IF、WB中进行了验证。 此纯化的mAb对磷酸组蛋白H2A.X（Ser139）（也被称为H2AXS139p）具有高度特异性。
研究子类别
组蛋白
研究类别
表观遗传学&核功能

联系

替代品：MABE205

外形

免疫亲和纯化的免疫球蛋白，溶于0.1M Tris-甘氨酸（pH 7.4）、0.15M NaCl、和0.05％叠氮化钠作为防腐剂的缓冲液中。
形式：纯化
蛋白G纯化

制备说明

自发货之日起在28°C可稳定保存1年。 为了最大程度地回收产品，在取下盖子之前，将原始样品瓶进行离心。

分析说明

免疫印迹分析：0.05-1 μg/ml的该抗体在5 μM星形孢菌素（货号19-123）处理过的Jurkat细胞中酸提取的组蛋白裂解物中检测到磷酸化的组蛋白H2A.X（Ser139）。
免疫细胞化学：2 μg/ml该抗体在用0.5 μM星形孢菌素处理4-6小时的HeLa细胞中检测到磷酸化的组蛋白H2A.X。
对照
紫外线处理的293细胞提取物、紫外线处理的HeLa细胞提取物或乳腺癌组织

其他说明

浓度：请参考批次特异性浓缩物的分析证书。

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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