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Merck
CN

05-850

Anti-p68 Antibody, clone204

clone 204, Upstate®, from mouse

别名:

Anti-G17P1, Anti-HUMP68, Anti-p68

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
204, monoclonal
Application:
western blot
Species reactivity:
human, rat, mouse
Citations:
24
Technique(s):
western blot: suitable
Uniprot accession no.:
P17844

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产品名称

Anti-p68 Antibody, clone204, clone 204, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

204, monoclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1

NCBI accession no.

NM_004396

UniProt accession no.

P17844

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... DDX5(1655)

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Routinely evaluated by immunoblot of nuclear extracts from HeLa cells

Application

Detect p68 with Anti-p68 Antibody, clone204 (Mouse Monoclonal Antibody), that has been shown to work in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

RNA Binding Protein (RBP)

Biochem/physiol Actions

p68

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

68kDa
Also known as DDX5, p68 is a DEAD-box ATPase and RNA splicing factor.

Immunogen

Purified T antigen-related protein from Ad2+ ND2 virus-infected HeLa cells.

Physical form

Format: Purified
Protein G Purified

Preparation Note

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1

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Kengo Takeda et al.
International journal of molecular sciences, 25(7) (2024-04-13)
Chronic myeloid leukemia (CML) is induced by the expression of the fused tyrosine kinase BCR-ABL, which is caused by a chromosomal translocation. BCR-ABL inhibitors have been used to treat CML; however, the acquisition of resistance by CML cells during treatment
Raphaël Métivier et al.
Cell, 115(6), 751-763 (2003-12-17)
Transcriptional activation of a gene involves an orchestrated recruitment of components of the basal transcription machinery and intermediate factors, concomitant with an alteration in local chromatin structure generated by posttranslational modifications of histone tails and nucleosome remodeling. We provide here
Zhi-Ren Liu
Molecular and cellular biology, 22(15), 5443-5450 (2002-07-09)
Modulation of the interaction between U1 snRNP and the 5' splice site (5'ss) is a key event that governs 5'ss recognition and spliceosome assembly. Using the methylene blue-mediated cross-linking method (Z. R. Liu, A. M. Wilkie, M. J. Clemens, and
M J Ford et al.
Nature, 332(6166), 736-738 (1988-04-21)
The p68 protein is a highly conserved nuclear antigen that is thought to be important in the regulation of cell growth and division. It is found in dividing cells of all mammals and amphibians tested, but not in quiescent cells.
Sumanta K Pal et al.
ACS omega, 4(2), 2472-2481 (2019-03-16)
Pyrvinium was identified as the first small molecule inhibitor of the androgen receptor (AR) DNA-binding domain (DBD). It was also among the first small molecules shown to directly inhibit the activity of AR splice variants (ARVs), which has important clinical
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相关内容

RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

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