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Merck
CN

07-436

抗单甲基组蛋白H3（Lys4）抗体

Upstate®, from rabbit

别名:

H3K4me1, Histone H3 (mono methyl K4), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗单甲基组蛋白H3（Lys4）抗体, Upstate®, from rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, vertebrates

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
inhibition assay: suitable (peptide)
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

monomethylation (Lys4)

Quality Level

100

Gene Information

human ... H3C1(8350)

相关类别

Application

研究类别
表观遗传学&核功能
免疫细胞化学：
由独立实验室报告。

肽抑制：
通过在HeLa酸提取物的免疫印迹分析中1 μM免疫肽完全消除组蛋白H3检测的能力，证实了特异性。（见图，泳道3）。含有二甲基赖氨酸4，三甲基赖氨酸4，单甲基赖氨酸4或单甲基赖氨酸9或27的肽未观察到竞争。
抗单甲基组蛋白H3（Lys4）抗体是一种兔多克隆抗体，用于检测单甲基组蛋白H3（Lys4）（也称为H3K4me1，组蛋白H3（单甲基K4）），&已在ICC、IF、PIA、WB、ChIP-seq、DB中得到验证。
研究子类别
组蛋白

Analysis Note

对照
HeLa细胞提取物
通过蛋白质印迹对HeLa细胞中酸提取的蛋白质进行了常规评估。

蛋白质印迹分析：
0.5-2 μg/mL该批次在HeLa细胞的酸提取蛋白质中检测到甲基化组蛋白H3。

Biochem/physiol Actions

识别单甲基化组蛋白H3（Lys4），Mr 17kDa。
预计具有广泛的物种交叉反应性。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

组蛋白H3是参与真核细胞染色质结构的五种主要组蛋白之一。H3具有一个主要的球状结构域和一个长N末端的尾巴，其与核糖体核小体′串珠′结构体的结构有关。组蛋白H3的N末端尾部从球状核小体核心突起，其可能发生几种不同类型的表观遗传修饰，从而影响细胞过程。这些修饰包括甲基或乙酰基与赖氨酸和精氨酸的共价连接，以及丝氨酸或苏氨酸的磷酸化。
17 kDa

Immunogen

KLH缀合的合成肽，包含序列ART[meK]…，其中meK对应于人组蛋白H3残基4处的单甲基赖氨酸。
表位：Lys4

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Physical form

形式：纯化
纯化的兔IgG，溶于含有0.1M Tris甘氨酸（pH 7.4）、0.15M NaCl、0.05％叠氮化钠的缓冲液中。
蛋白A纯化

Preparation Note

自收到之日起，在2至8°C条件下可稳定保存1年。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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