biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, vertebrates
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, inhibition assay: suitable (peptide), western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
monomethylation (Lys4)
Gene Information
human ... H3C1(8350)
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General description
组蛋白H3是参与真核细胞染色质结构的五种主要组蛋白之一。H3具有一个主要的球状结构域和一个长N末端的尾巴,其与核糖体核小体′串珠′结构体的结构有关。组蛋白H3的N末端尾部从球状核小体核心突起,其可能发生几种不同类型的表观遗传修饰,从而影响细胞过程。这些修饰包括甲基或乙酰基与赖氨酸和精氨酸的共价连接,以及丝氨酸或苏氨酸的磷酸化。
17 kDa
Immunogen
KLH缀合的合成肽,包含序列ART[meK]…,其中meK对应于人组蛋白H3残基4处的单甲基赖氨酸。
表位:Lys4
Application
免疫细胞化学:
由独立实验室报告。
肽抑制:
通过在HeLa酸提取物的免疫印迹分析中1 μM免疫肽完全消除组蛋白H3检测的能力,证实了特异性。(见图,泳道3)。含有二甲基赖氨酸4,三甲基赖氨酸4,单甲基赖氨酸4或单甲基赖氨酸9或27的肽未观察到竞争。
由独立实验室报告。
肽抑制:
通过在HeLa酸提取物的免疫印迹分析中1 μM免疫肽完全消除组蛋白H3检测的能力,证实了特异性。(见图,泳道3)。含有二甲基赖氨酸4,三甲基赖氨酸4,单甲基赖氨酸4或单甲基赖氨酸9或27的肽未观察到竞争。
抗单甲基组蛋白H3(Lys4)抗体是一种兔多克隆抗体,用于检测单甲基组蛋白H3(Lys4)(也称为H3K4me1,组蛋白H3(单甲基K4)),&已在ICC、IF、PIA、WB、ChIP-seq、DB中得到验证。
研究子类别
组蛋白
组蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
识别单甲基化组蛋白H3(Lys4),Mr 17kDa。
预计具有广泛的物种交叉反应性。
Physical form
形式:纯化
纯化的兔IgG,溶于含有0.1M Tris甘氨酸(pH 7.4)、0.15M NaCl、0.05%叠氮化钠的缓冲液中。
蛋白A纯化
Preparation Note
自收到之日起,在2至8°C条件下可稳定保存1年。
Analysis Note
对照
HeLa细胞提取物
HeLa细胞提取物
通过蛋白质印迹对HeLa细胞中酸提取的蛋白质进行了常规评估。
蛋白质印迹分析:
0.5-2 μg/mL该批次在HeLa细胞的酸提取蛋白质中检测到甲基化组蛋白H3。
蛋白质印迹分析:
0.5-2 μg/mL该批次在HeLa细胞的酸提取蛋白质中检测到甲基化组蛋白H3。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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