Gab1 phosphorylated on Tyrosine residue 627.

Predicted to cross-react with human based on 100% sequence homology, but have not been tested.

Peptide corresponding to amino acid region encompassing the human and mouse phospho-Gab1 (Tyr627)

Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Gab1 (Tyr627) in RIPA lysate of PDGF treated NIH3T3 cells.

Research Category
Signaling

Research Sub Category
MAP Kinases

This Anti-phospho-Gab1 (Tyr627) Antibody is validated for use in WB for the detection of phospho-Gab1 (Tyr627).

Routinely evaluated by immunoblot.

115 kDa

Affinity purified

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

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