07-833
Anti-phospho-Gab1 (Tyr627) Antibody
Upstate®, from rabbit
别名:
GAB1, GRB2-associated binding protein 1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
mouse
Application:
WB
Citations:
8
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pTyr627)
Quality Level
Gene Information
mouse ... Gab1(14388)
General description
115 kDa
Immunogen
Peptide corresponding to amino acid region encompassing the human and mouse phospho-Gab1 (Tyr627)
Application
This Anti-phospho-Gab1 (Tyr627) Antibody is validated for use in WB for the detection of phospho-Gab1 (Tyr627).
Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Gab1 (Tyr627) in RIPA lysate of PDGF treated NIH3T3 cells.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Biochem/physiol Actions
Gab1 phosphorylated on Tyrosine residue 627.
Predicted to cross-react with human based on 100% sequence homology, but have not been tested.
Physical form
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Affinity purified
Preparation Note
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
C Boccaccio et al.
Nature, 391(6664), 285-288 (1998-01-24)
Hepatocyte growth factor (HGF) induces a three-phase response leading to the formation of branched tubular structures in epithelial cells. The HGF receptor tyrosine kinase works through a Src homology (SH2) docking site that can activate several signalling pathways. The first
M Shinohara et al.
The Journal of biological chemistry, 276(22), 18941-18946 (2001-03-23)
Gab-1 is a multiple docking protein that is tyrosine phosphorylated by receptor tyrosine kinases such as c-Met, hepatocyte growth factor/scatter factor receptor, and epidermal growth factor receptor. We have now demonstrated that cell-cell adhesion also induces marked tyrosine phosphorylation of
Lilly Y W Bourguignon et al.
The Journal of biological chemistry, 278(32), 29420-29434 (2003-05-16)
In this study we have examined CD44 (a hyaluronan (HA) receptor) interaction with a RhoA-specific guanine nucleotide exchange factor (p115RhoGEF) in human metastatic breast tumor cells (MDA-MB-231 cell line). Immunoprecipitation and immunoblot analyses indicate that both CD44 and p115RhoGEF are
M Holgado-Madruga et al.
Nature, 379(6565), 560-564 (1996-02-08)
The protein Grb2 plays a central role in signalling by receptor protein-tyrosine kinases, where its SH2 domain binds to the receptor and its two SH3 domains link to effectors. One target effector is Sos, so Grb2 links receptor protein-tyrosine kinases
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