09-774
Anti-EED Antibody
from rabbit
别名:
embryonic ectoderm development
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
western blot
Species reactivity:
human, mouse
Citations:
42
Technique(s):
western blot: suitable
Uniprot accession no.:
产品名称
Anti-EED Antibody, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
species reactivity (predicted by homology)
dog, chicken, bovine, opossum, rat
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
dog ... Eed(476779)
human ... EED(8726)
mouse ... Eed(13626)
rat ... Eed(293104)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 09-727
Physical form
Format: Purified
Analysis Note
Control
Jurkat Cell Lysate
3T3/A31 cell lysate
Jurkat Cell Lysate
3T3/A31 cell lysate
Western Blot Analysis: 1 μg/mL of this lot detected EED on 10 μg of Jurkat cell lysate.
Application
Use Anti-EED Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EED also known as embryonic ectoderm development.
Biochem/physiol Actions
This antibody recognizes EED, Mr ~ 62-70 kDa.
General description
Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.
~53 kDa observed. Uniprot describes three isoforms at 51 kDa (isoform 1), 53 kDa (isoform 2), and 46 kDa (isoform 3).
Immunogen
A synthetic peptide corresponding to a.a. 429-441 of human EED, conjugated to KLH.
Epitope: a.a. 429-441
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Falak Sher et al.
Cellular reprogramming, 13(1), 1-6 (2010-10-29)
Recently, we have demonstrated the expression of the polycomb group protein Ezh2 in embryonic and adult neural stem cells. Although Ezh2 remained highly expressed when neural stem cells differentiate into oligodendrocyte precursor cells, it is downregulated during the differentiation into
Sylvia Mahara et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(26), E3735-E3744 (2016-06-16)
Despite the established oncogenic function of Polycomb repressive complex 2 (PRC2) in human cancers, its role as a tumor suppressor is also evident; however, the mechanism underlying the regulation of the paradoxical functions of PRC2 in tumorigenesis is poorly understood.
Bin Shi et al.
Cancer medicine, 8(14), 6383-6392 (2019-08-29)
The aims of this study were to investigate the link between enhancer of zeste homolog 2 (EZH2) and histone deacetylase (HDAC) in preclinical studies and in human lung cancer tissue microarrays. Enhancer of zeste homolog 2 and HDAC1 mRNA expression
Taichi Takashina et al.
Cancer science, 107(7), 955-962 (2016-04-27)
Recent discoveries have revealed that human cancer involves aberrant epigenetic alterations. We and others have previously shown that the histone methyltransferase EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), is frequently overexpressed in non-small-cell lung cancer (NSCLC) and
Stefanie Göllner et al.
Nature medicine, 23(1), 69-78 (2016-12-13)
In acute myeloid leukemia (AML), therapy resistance frequently occurs, leading to high mortality among patients. However, the mechanisms that render leukemic cells drug resistant remain largely undefined. Here, we identified loss of the histone methyltransferase EZH2 and subsequent reduction of
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