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Merck
CN

16-201

蛋白质A琼脂糖/鲑鱼精子DNA,2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

别名:

ChIP agarose beads, ChIP agaraose G beads

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Form:
liquid
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Quality Level

form

liquid

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable

suitability

suitable for immunoprecipitation

shipped in

wet ice

General description

重组蛋白G对IgG分子的Fc区具有非常高的亲和力。将蛋白G固定在微珠上会形成一种亲和树脂,可用于从粗血清、腹水或细胞培养基中纯化IgG组分。
重组蛋白G通过溴化氰键与琼脂糖共价结合。当收集用于染色质免疫沉淀(ChIP分析)的免疫复合物时,超声处理的鲑鱼精子DNA会阻断蛋白G琼脂糖上的非特异性DNA结合位点。

Packaging

2.5 mL填充珠以50%凝胶浆形式提供,最终体积为5 mL。

Physical form

含有1 mg超声鲑鱼精子DNA、2.5 mg BSA和约5 mg重组蛋白G的2.5 mL填充珠,以50%凝胶浆形式提供,每瓶最终体积为5 mL。 混悬在含有0.05%叠氮化钠的10 mM Tris-HCl,1 mM EDTA(pH 8.0)中。 液体混悬液。

Preparation Note

自发货之日起在4°C下可保存1年。

Analysis Note

在染色质免疫沉淀中常规评估,用于预先清除丁酸钠处理的HeLa细胞的上清液,以及随后与乙酰化组蛋白交联的DNA的免疫沉淀

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。


存储类别

10 - Combustible liquids

wgk

WGK 1



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相关内容

蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

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全球贸易项目编号

货号GTIN
16-20104053252326370