17-128
Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A)
Alkaline/Acid Phosphatase Assay Kit is routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP.
应用
Used to detect/quantify: Alkaline/Acid
包装
Kit capacity: 100 assays
分析说明
routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP
其他说明
Malachite Green Solution A (Cat.# 20-105)
Malachite Green Additive (Cat.# 20-104)
Phosphate Standard (Cat.# 20-103)
Serine Phosphopeptide (RRApSVA) (Cat.# 12-220)
pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
NiCl2, 40mM (Cat.# 20-178)
pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
96-well microtiter plate
Malachite Green Additive (Cat.# 20-104)
Phosphate Standard (Cat.# 20-103)
Serine Phosphopeptide (RRApSVA) (Cat.# 12-220)
pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
NiCl2, 40mM (Cat.# 20-178)
pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
96-well microtiter plate
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语：
Danger
危险分类
Aquatic Chronic 3 - Carc. 1A Inhalation - Met. Corr. 1 - Repr. 1B - Skin Sens. 1 - STOT RE 2
靶器官
Lungs
储存分类代码
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
法规信息
新产品
此项目有
Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green.
Ekman, P and Jager, O
Analytical biochemistry, 214, 138-141 (1993)
Nathan J Castro et al.
Tissue engineering. Part A, 22(13-14), 940-948 (2016-06-15)
Osseous tissue defects caused by trauma present a common clinical problem. Although traditional clinical procedures have been successfully employed, several limitations persist with regards to insufficient donor tissue, disease transmission, and inadequate host-implant integration. Therefore, this work aims to address
An investigation of the substrate specificity of protein phosphatase 2C using synthetic peptide substrates; comparison with protein phosphatase 2A
Donella Deana, A., et al
Biochimica et Biophysica Acta, 1051, 199-202 (1990)
A Donella-Deana et al.
Biochimica et biophysica acta, 1094(1), 130-133 (1991-08-13)
The four main classes of protein phosphatases (PP-1, 2A, 2B and 2C), although differing in their ability to dephosphorylate phosphopeptide substrates, invariably display a marked preference toward phosphothreonyl peptides over their phosphoseryl counterparts. Conversely, all the acidic and alkaline phosphatases
P P Van Veldhoven et al.
Analytical biochemistry, 161(1), 45-48 (1987-02-15)
A procedure, based on the complex formation of malachite green with phosphomolybdate under acidic conditions, to measure inorganic orthophosphate in the nanomolar range is described. The addition of polyvinyl alcohol is required to stabilize the dye-phosphomolybdate complex. The advantages of
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