Cholera Toxin, B Subunit, Vibrio cholerae, Type Inaba 569B - Calbiochem

Cholera toxin is an oligomeric protein consisting of a single A subunit (M.W. 28,000) containing two polypeptide chains, A1 and A2 , and a B subunit (M.W. 55,000) containing five polypeptide chains. Cholera toxin is the pathogenic agent responsible for the symptoms of cholera. Cholera toxin is also a potent activator of adenylate cyclase that binds the ADP-ribosylation factor and modulates its activity. The A subunit catalyzes the ADP-ribosylation of GS that leads to the persistent activation of adenylate cyclase. The consequent increase in intracellular cAMP levels stimulates Cl- secretion and leads to severe dehydration. The B subunit binds specifically to GM₁ ganglioside receptors, which are expressed ubiquitously in mammalian cells, and assists in the transport of the A subunit through the membrane. When used alone, the B subunit will block the action of toxin on intact cells. Purified B subunit is produced by a modification of the methods published by Lai, C.Y., et al.

Subunit of cholera toxin responsible for binding to GM₁ ganglioside receptors in membranes. When used along, will block the action of toxin on intact cells.

Cell permeable: no

Cholera Toxin, B Subunit is tested for its effectiveness in binding antitoxin using the antitoxin combining power assay described by Craig, J.P., et al.

Primary Target
GM1 ganglioside receptors

Reversible: no

yes

Lyophilized from 200 mM NaCl, 50 mM Tris-HCl, 3 mM NaN₃, 1 mM EDTA, pH 7.5.

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C. DO NOT FREEZE reconstituted solutions.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

McCloskey, M.A. and Zhang, L. 2000. J. Cell. Biol.148, 137.
Mulhein, S.A., et al. 1989. J. Membr. Biol. 109, 21.
Tsai, S.-C., et al. 1988. J. Biol. Chem.263, 1768.
Van Heynigen, S. 1982. Biosci. Rep.2, 135.
Mekalanos, J.J., et al. 1978. Infect. Immun.20, 552.
Gill, D.M. 1976. Biochemistry15, 1242.
Lai, C.Y., et al. 1976. J. Infect. Dis.139, 674.
Rappaport, R.S., et al. 1974. Infect. Immun.9, 294.
Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.

Not available for sale outside of the United States.

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Toxicity: Highly Toxic (H)