Exoenzyme C3, Clostridium botulinum

Catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn⁴¹ in Rho. Inhibits the progression of the cell cycle from G₁ to S phase.

Catalyzes the hydrolysis of NAD. Can be used as a reagent for the detection of Rho proteins. Predominantly catalyzes the ADP ribosylation of the Asn⁴¹ amino acid residue in Rho. Treatment of cultured cells with exoenzyme C3 has been shown to induce ADP-ribosylation of RhoA protein and to reduce cell growth rate and saturation density. Its growth inhibitory effects appear to result from blockage at the G₁ to S phase transition, causing the accumulation of cells in the G₁ phase.

Exoenzyme C3, Clostridium botulinum, CAS 58319-92-9, catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho.

Lyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.

Reconstitute with sterile distilled H₂O

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CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Irritant (B)