GAG Agonist, Adhesamine - Calbiochem

A symmetrical nonpeptidyl cationic diaryldispirotripiperazine compound that binds GAGs (glycosaminoglycans) in a reversible manner (K_D = 0.39, 4.67, and 5.85 μM for heparin, heparan sulfate, and keratin sulfate, respectively) and effectively promotes adhesion (by ~100 and 600% at 60 μM, respectively) and growth (by ~80 and 50% in 72 h at 1 μM, respectively) of HepG2 and Jurkat cells in a dose-dependent manner. Binding studies using heparin-derived oligosaccharides indicate that the major mode of interaction involves the binding of a hexasaccharide unit by two adhesamine molecules (K_D = 0.12 μM). Primary murine hippocampal neurons cultured in the presence of adhesamine are reported to exhibit earlier differentiation as well as improved survival rate when compared with neurons cultured on poly-L-lysine-coated surface. A GAGs binding antagonist Surfen is also available under Cat. No. 362330.

The GAG Agonist, Adhesamine controls the biological activity of GAG. This small molecule/inhibitor is primarily used for Activators/Inducers applications.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.