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Merck
CN

371723

Sigma-Aldrich

Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355) Rabbit pAb

liquid, Calbiochem®

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UNSPSC代码:
12352203
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生物来源

rabbit

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

liquid

不包含

preservative

种属反应性(根据同源性预测)

mammals

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG

运输

wet ice

储存温度

−70°C

靶向翻译后修饰

unmodified

基因信息

human ... GNAI1(2770)

一般描述

Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355), rabbit polyclonal, recognizes Giα-1 and Giα-2 subunits. It is validated for use in Western blotting.
Protein A purified rabbit polyclonal antibody. Recognizes Giα-1 and Giα-2 subunit proteins.
Recognizes Giα-1 and Giα-2 subunits. Does not cross-react with G3α-3 or Goα subunits.

免疫原

a synthetic peptide [(C)KNNLKDCGLF] corresponding to amino acids at the C-terminus of mammalian Giα-1 and Giα-2, conjugated to KLH

应用

Immunoblotting (1:1000)

外形

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

制备说明

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Does not cross-react with Giα-3 and Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Toxicity: Standard Handling (A)

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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K Takano et al.
Endocrinology, 138(6), 2405-2409 (1997-06-01)
SRIF activates an inwardly rectifying K+ current in human GH-secreting adenoma cells. Activation of this K+ current induces hyperpolarization of the membrane and abolishment of action potential firing. This mechanism is an essential mechanism for SRIF-induced decrease in intracellular Ca2+
C Puma et al.
Journal of neurochemistry, 78(5), 960-971 (2001-09-13)
The chemokine IL-8 is known to be synthesized by glial cells in the brain. It has traditionally been shown to have an important role in neuroinflammation but recent evidence indicates that it may also be involved in rapid signaling in
Y Xu et al.
Infection and immunity, 64(2), 593-599 (1996-02-01)
In previous studies, an in vitro ADP-ribosylation assay was developed to quantitatively evaluate the in vivo ADP-ribosylation of eukaryotic target proteins in intact Chinese hamster ovary (CHO) cells by pertussis toxin (PT). Immunoblot analysis identified the two PT-sensitive target proteins
Jennifer S Ignatious Raja et al.
The European journal of neuroscience, 39(8), 1245-1255 (2014-01-22)
Intracellular signaling in insect olfactory receptor neurons remains unclear, with both metabotropic and ionotropic components being discussed. Here, we investigated the role of heterotrimeric Go and Gi proteins using a combined behavioral, in vivo and in vitro approach. Specifically, we
K Takano et al.
The Journal of physiology, 502 ( Pt 3), 559-567 (1997-08-01)
1. Types of G proteins (G protein alpha-subunit subtypes) which mediate the activation of inward rectifier K+ currents by somatostatin (somatotrophin release-inhibiting factor, SRIF) were determined in cultured locus coeruleus neurones from newborn rats and in AtT-20 cells (a mouse

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