Hh Signaling Antagonist XI, MRT-14

A cell-permeable acylurea compound that acts as an antagonistic Smo ligand (IC₅₀ = 120 nM against 5 nM Bodipy-cyclopamine binding to murine Smo-transfected HEK293 cells) and is more effective than Cyclopamine (Cat. No. 239803 ) in inhibiting ShhN-induced Gli transcription in Shh-light 2 cell assays (IC₅₀ = 0.16 and 0.3 µM, respectively) and in blocking 0.1 µM SAG- (Cat. Nos. 566660 & 566661) stimulated differentiation of C3H10T1/2 murine mesenchymal stem cells into osteoblasts (IC₅₀ = 0.13 and 0.62 µM, respectively).

The Hh Signaling Antagonist XI, MRT-14 controls the biological activity of Hh pathway. This small molecule/inhibitor is primarily used for Cell Signaling applications.

Packaged under inert gas

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Manetti, F., et al. 2010. Mol. Pharmacol.78, 658.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)