382159-M
PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb
liquid, Calbiochem®
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans
Application:
—
Citations:
-
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer10)
Quality Level
Gene Information
human ... H3C1(8350)
General description
Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
This PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).
Immunogen
Human
a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
Application
Immunoblotting (0.5 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunohistochemistry (see comments)
Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
Immunocytochemistry (5 µg/ml)
Immunohistochemistry (see comments)
Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
Physical form
In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Acid-extracted proteins from colcemid-arrested HeLa cells
Acid-extracted proteins from colcemid-arrested HeLa cells
Other Notes
Ajiro, K., et al. 1996. J. Biol. Chem.271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.
For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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