540220
嘌吗啡胺
≥98% (HPLC), solid, Hedgehog signaling activator, Calbiochem
别名:
嘌吗啡胺
Product Name
嘌吗啡胺, A cell-permeable activator of Hedgehog signaling that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM).
方案
≥98% (HPLC)
表单
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
protect from light
颜色
off-white
溶解性
DMSO: 200 mg/mL
运输
ambient
储存温度
2-8°C
SMILES字符串
N7(CCOCC7)c1ccc(cc1)Nc2nc(nc5[n](cnc52)C6CCCCC6)Oc3c4c(ccc3)cccc4
InChI
1S/C31H32N6O2/c1-2-9-25(10-3-1)37-21-32-28-29(33-23-13-15-24(16-14-23)36-17-19-38-20-18-36)34-31(35-30(28)37)39-27-12-6-8-22-7-4-5-11-26(22)27/h4-8,11-16,21,25H,1-3,9-10,17-20H2,(H,33,34,35)
InChI key
FYBHCRQFSFYWPY-UHFFFAOYSA-N
一般描述
一种刺猬信号的细胞渗透激活剂，可诱导多能间充质祖细胞C3H10T1/2（EC₅₀=1 µM）的成骨细胞分化。
一种细胞可渗透的2,6,9-三取代嘌呤化合物，可诱导多能间充质祖细胞C3H10T1/2（EC50=1 µM）和谱系固定的前成骨细胞MC3T3-E1的成骨细胞分化。它的作用可以与BMP-4（骨形态发生蛋白-4）的作用协同，并且已显示即使在前脂肪细胞和成肌细胞中也能诱导转分化。据报道通过激活刺猬信号通路诱导成骨。也可提供50 mM (5 mg/192 µl)2,6,9-三元取代嘌呤（目录号540223）在DMSO中的溶液。
请注意，由于水含量的变化，该化合物的分子量是因批次特异性的。
请注意，由于水含量的变化，该化合物的分子量是因批次特异性的。
一种细胞渗透性嘌呤化合物，可在C3H10T1/2多能间充质祖细胞（EC50 = 1 µM）和MC3T3-E1谱系中诱导成骨细胞分化。还显示在前脂肪细胞和成肌细胞中诱导转分化。据报道，其作用与骨形态发生蛋白-4（BMP-4）具有协同作用。据报道通过激活刺猬信号通路诱导成骨。
生化/生理作用
EC50 = 1 µM，用于诱导多能间充质祖细胞C3H10T1/2的成骨细胞分化
主靶
刺猬信号通路的激活
刺猬信号通路的激活
产物不与ATP竞争。
细胞可渗透性：是
制备说明
复溶后，等分并冷冻保存（-20°C）。贮备溶液在-20°C下可稳定保存至多3个月。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
毒性：有害（C）
警示用语：
Warning
危险声明
危险分类
Muta. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Hynek Wichterle et al.
Methods in molecular biology (Clifton, N.J.), 482, 171-183 (2008-12-18)
A growing number of specific cell types have been successfully derived from embryonic stem cells (ES cells), including a variety of neural cells. In vitro generated cells need to be extensively characterized to establish functional equivalency with their in vivo
Hynek Wichterle et al.
Current protocols in stem cell biology, Chapter 1, Unit 1H-Unit 1H (2008-09-05)
Controlled differentiation of embryonic stem (ES) cells into clinically relevant cell types is a fundamental goal of stem cell research. This unit describes one of the most efficient protocols for conversion of mouse ES cells into a defined type of
Yusuke Naito et al.
SLAS discovery : advancing life sciences R & D (2023-06-28)
The beta-glucocerebrosidase (GBA1) gene encodes the lysosomal beta-glucocerebrosidase (GCase) that metabolizes the lipids glucosylceramide (GlcCer) and glucosylsphingosine (GlcSph). Biallelic loss-of-function mutations in GBA1 such as L444P cause Gaucher disease (GD), which is the most prevalent lysosomal storage disease and is
Tetsushi Kataura et al.
Journal of neurochemistry, 155(1), 81-97 (2020-03-05)
Bilirubin, the end product of heme redox metabolism, has cytoprotective properties and is an essential metabolite associated with cardiovascular disease, inflammatory bowel disease, type 2 diabetes, and neurodegenerative diseases including Parkinson's disease (PD). PD is characterized by progressive degeneration of
High-content screening of mitochondrial polarization in neural cells derived from human pluripotent stem cells.
Zink, et al.
STAR protocols, 3, 101602-101602 (2022)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持