Anti-HLA-ABC Common Antibody, clone 22.63.4

BIOCHEMISTRY: p I = 7.8

The previously assigned protein identifier P16189 has been merged into P04439. Full details can be found on the UniProt database.

Nalm-6 cell line

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-HLA-ABC Common Antibody, clone 22.63.4 is validated for use in FC, IC, IH for the detection of HLA-ABC.

This antibody is suitable for indirect immunofluorescence analysis by flow cytometry and is an excellent positive control. It is suitable for cryostat sections but not for routine formalin fixed paraffin sections. If the tissue is prepared at 4°C and fixed in PLP (Periodate-Lysine-Paraformaldehyde) before paraffin embedding, the antigen can be conserved (Hancock, 1982).

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:15 - 1:60

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:15 will be applicable to most commonly used systems.

This antibody reacts with a common framework determinant of the 40-44kD protein of HLA-ABC Class I antigen. The antibody precipitates antigens of 40 - 44kD and 12kD (b2-microglobulin).

Cell reactivity:

The antibody reacts with all cells having HLA-ABC surface antigen. Erythrocytes and K562 and DAUDI are negative.

Format: Purified

Purified from mouse ascitic fluid. The antibody is supplied in phosphate buffered saline, pH 7.4 containing 0.1% sodium azide, diluted 50% v/v with glycerol. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store at -15 to -20°C in undiluted aliquots. At this temperature the glycerol solution will not freeze.

WARNING: The monoclonal reagent solution contains 0.05% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MAB1280

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