生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
BnC6B4, monoclonal
种属反应性
chicken
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... ITGA6(3655)
特异性
Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.
免疫原
Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6M guanidine isothiocyanate, and depleted of beta1 subunit.
应用
Anti-Integrin α6 Antibody, clone BnC6B4 is an antibody against Integrin α6 for use in IP, WB, IC, IH.
Western blot
Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.
Immunohistochemistry: Effective for immunohistochemistry of methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse Ig -FITC secondary antiobody.
Immunocytochemistry: Effective for methanol-fixed cultured cells (15 minute fixation).
Immunoblot: Recognizes a 130 kDa band in chicken brain tissue and a 140 kDa band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).
Optimal working dilutions must be determined by end user.
Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.
Immunohistochemistry: Effective for immunohistochemistry of methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse Ig -FITC secondary antiobody.
Immunocytochemistry: Effective for methanol-fixed cultured cells (15 minute fixation).
Immunoblot: Recognizes a 130 kDa band in chicken brain tissue and a 140 kDa band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).
Optimal working dilutions must be determined by end user.
外形
Format: Purified
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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