biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
H1, monoclonal
species reactivity
rat, human, bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, radioimmunoassay: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... KIF5B(3799)
Immunogen
表位:重链,非神经元KHC
Application
在多聚甲醛固定的组织上进行免疫组化。也适用于EM。
免疫细胞化学:多聚甲醛固定的细胞; 5-10′ RT,新鲜制备的4% PFA;以 0.1-.2% triton X-100透化的3% 正常血清封闭缓冲液
免疫印迹:识别非神经元样品的 124kDa KHC
免疫沉淀
驱动蛋白介导的转运的抑制
EIA/RIA
最佳工作稀释度必须由最终用户进行确定。
免疫细胞化学:多聚甲醛固定的细胞; 5-10′ RT,新鲜制备的4% PFA;以 0.1-.2% triton X-100透化的3% 正常血清封闭缓冲液
免疫印迹:识别非神经元样品的 124kDa KHC
免疫沉淀
驱动蛋白介导的转运的抑制
EIA/RIA
最佳工作稀释度必须由最终用户进行确定。
抗驱动蛋白抗体(重链,非神经元KHC,克隆H1)可检测驱动蛋白水平 & 已有相关文献发表 & 经验证可用于ELISA、IP、RIA、WB、IC、IH。
Biochem/physiol Actions
与非神经元 KHC的重链N末端附近的的主要形式反应。
Physical form
形式:纯化
液体形式,溶于0.02 M磷酸盐缓冲液、0.25 M NaCl(pH 7.6)和0.1%叠氮化钠中。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Sten Strunze et al.
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Many viruses deliver their genomes into the host cell nucleus for replication. However, the size restrictions of the nuclear pore complex (NPC), which regulates the passage of proteins, nucleic acids, and solutes through the nuclear envelope, require virus capsid uncoating
Hongtao Chen et al.
Biophysical journal, 94(4), 1508-1520 (2007-11-06)
The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that
Wen-Jun Wang et al.
Drug metabolism and disposition: the biological fate of chemicals, 42(1), 62-69 (2013-10-12)
Previous studies identified a family of organic anion transport proteins (OATPs), many of which have C-terminal PDZ binding consensus sequences. In particular, the C-terminal four amino acids of Oatp1a1, a transporter on rat and mouse hepatocytes, comprise a consensus binding
Souvik Sarkar et al.
Traffic (Copenhagen, Denmark), 7(8), 1078-1091 (2006-06-01)
Intracellular trafficking regulates the abundance and therefore activity of transporters present at the plasma membrane. The transporter, Na+-taurocholate co-transporting polypeptide (ntcp), is increased at the plasma membrane upon treatment of cells with cAMP, for which microtubules (MTs) are required and
Alexander S Zhovmer et al.
ACS nano, 15(11), 17528-17548 (2021-10-23)
Microtubules (MTs) and MT motor proteins form active 3D networks made of unstretchable cables with rod-like bending mechanics that provide cells with a dynamically changing structural scaffold. In this study, we report an antagonistic mechanical balance within the dynein-kinesin microtubular
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