Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor^™488 conjugated

The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.

Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987).

Flow Cytometry: 1-3 μg LM609 per 0.5 x 10e6 cells in 100μL (Smith, 1996).Immunoprecipitation: Specifically immunoprecipitates the integrin alphaVbeta3 complex which, when resolved by SDS-PAGE under reducing conditions, results in an approximately 130 kDa band corresponding to the alphaV subunit and a 105 kDa band corresponding to the beta3 subunit (Cheresh, 1987).

Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections.

Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor^™488 conjugated is validated for use in FC, IF for the detection of Integrin αVβ3.

Reacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.

Protein A purified

Purified immunoglobulin from Protein A Sepharose^™ chromatography, conjugated to Alexa Fluor 488. Final formulation in PBS 0.01M, SODIUM AZIDE 0.1%, PH7.1, 15mg/mL BSA.

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
POSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995).

NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV

Chen, 1995).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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