生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
LB9, monoclonal
种属反应性
mouse, rat, human
技术
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... CREB1(1385)
一般描述
Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation on by various kinases, including PKA and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.
特异性
Clone LB9 reactivity towarad spliced isoform CREB-B and htCREB (isoform 3) has not been determined.
应用
Anti-CREB Antibody, clone LB9, Ascites Free is an antibody against CREB for use in Western Blotting, Immunohistochemistry (Paraffin).
Western Blotting Analysis: 1.0 µg/mL of this antibody from a representative lot detected CREB in 10 µg of NIH/3T3 and PC12 cell lysate.
Immunohistochemistry Analysis: An 1:50-250 dilution of this antibody from a representative lot detected CREB in human liver, colon, and tonsil tissue sections.
Western Blotting Analysis: Representative lots detected CREB in primary mouse astrocytes and mouse dorsal hippocampal tissue lysates (Orr, A.G., et al. (2015). Nat. Neurosci. 18(3):423-434; Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
Western Blotting Analysis: Representative lots detected CREB in primary human fibroblast and SH-SY5Y human neuroblastoma cell lysates (Maj, M., et al. (2011). Mitochondrion. 11(2):264-272; Fukuda, Y., et al. (2010). Mol. Cell Neurosci. 45(3):226-233).
Immunohistochemistry Analysis: A representative lot immunostained total CREB in dorsal hippocampal tissue sections from paraformaldehyde-perfused mice (Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
Immunohistochemistry Analysis: An 1:50-250 dilution of this antibody from a representative lot detected CREB in human liver, colon, and tonsil tissue sections.
Western Blotting Analysis: Representative lots detected CREB in primary mouse astrocytes and mouse dorsal hippocampal tissue lysates (Orr, A.G., et al. (2015). Nat. Neurosci. 18(3):423-434; Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
Western Blotting Analysis: Representative lots detected CREB in primary human fibroblast and SH-SY5Y human neuroblastoma cell lysates (Maj, M., et al. (2011). Mitochondrion. 11(2):264-272; Fukuda, Y., et al. (2010). Mol. Cell Neurosci. 45(3):226-233).
Immunohistochemistry Analysis: A representative lot immunostained total CREB in dorsal hippocampal tissue sections from paraformaldehyde-perfused mice (Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
质量
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected CREB in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected CREB in 10 µg of HeLa cell lysate.
目标描述
~37-43 kDa observed. 36.69 kDa (isoform CREB-A) calculated.
外形
Format: Purified
其他说明
Concentration: Please refer to lot specific datasheet.
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持