MAB8878
Anti-Cdk1 Antibody, clone A17.1.1
clone A17.1.1, Chemicon®, from mouse
别名:
p34cdc2, CDC2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
A17.1.1, monoclonal
Application:
IF, IHC, IP, WB, activity assay
Citations:
7
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
A17.1.1, monoclonal
species reactivity
human, Xenopus, mouse, rat, chicken
manufacturer/tradename
Chemicon®
technique(s)
activity assay: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Cdk1(12534)
Immunogen
Raised against Xenopus p34cdc2.
Application
Anti-Cdk1 Antibody, clone A17.1.1 is a Mouse Monoclonal Antibody for detection of Cdk1 also known as p34cdc2, CDC2 & has been validated in IF, IP, EA, WB, IHC(P).
Frozen Sections
Immunofluorescence
Immunoprecipitation: (Native/denatured, Protein A; 2μg/mg protein lysate)
Immunohistology (Formalin, 2-4μg/mL for 30 min at RT)
Paraffin Sections (staining requires antigen retrieval).
Western Blotting
Kinase Assay
Optimal working dilutions must be determined by the end user.
p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. MAB8878 inhibits the activation of p34cdc2 kinase by cyclins and precipitates an active kinase.
Immunofluorescence
Immunoprecipitation: (Native/denatured, Protein A; 2μg/mg protein lysate)
Immunohistology (Formalin, 2-4μg/mL for 30 min at RT)
Paraffin Sections (staining requires antigen retrieval).
Western Blotting
Kinase Assay
Optimal working dilutions must be determined by the end user.
p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. MAB8878 inhibits the activation of p34cdc2 kinase by cyclins and precipitates an active kinase.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
Recognizes a 34 kDa protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks).
Physical form
Format: Purified
Purified immunoglobulin. 0.2 mg/mL in 10 mM PBS, pH 7.4, containing 0.2% BSA and 0.09% Sodium Azide.
Preparation Note
Maintain at 2° to 8°C in undiluted aliquots for up to 12 months from date of receipt.
Analysis Note
Control
POSITIVE CONTROL:
HeLa or NIH3T3 cells. Germinal center in a tonsil or reactive lymph node. Cellular localization is predominantly nuclear, with some cytoplasmic staining.
POSITIVE CONTROL:
HeLa or NIH3T3 cells. Germinal center in a tonsil or reactive lymph node. Cellular localization is predominantly nuclear, with some cytoplasmic staining.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Ian M McDonald et al.
The Journal of biological chemistry, 295(8), 2359-2374 (2020-01-04)
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