Anti-Oct-4 Antibody, clone 9B7

The POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

Recombinant protein corresponding to human Oct-4.

Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.

~39 kDa observed

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse embryonic stem cell lysate

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