Anti-HHV-8 LANA1 Antibody, clone 1D10C3

Human herpesvirus-8 (also known as Kaposi′s sarcoma-associated herpesvirus, HHV-8, KSHV) is etiologically associated with all forms of Kaposi sarcoma (KS) including classic, endemic, transplant-related, and AIDS-related, as well as rare neoplastic disorders. It is a double stranded DNA virus with a large genome that encodes for over 87 open reading frames (ORFs) including genes necessary for capsid, tegument, envelope, DNA replication and regulatory proteins. HHV-8 undergoes a bi-phasic lifecycle and can persist indefinitely in the infected host in a latent form during which time only a small fraction of regulatory viral proteins is expressed. The most prominent protein expressed in latently infected cells is the ORF73 gene product known as the latency-associated nuclear antigen (LANA) that functions to ensure the maintenance of the viral genome by tethering the viral episomal DNA to host cell chromosomes. It is a multi-functional protein that plays a role in the replication and long-term persistence of the viral episomal genome in dividing cells. It binds to mitotic chromosomes via its N-terminal region and to a 16-bp imperfect palindrome within the origin of replication (oriP) located in the viral terminal repeat (TR) through its C-terminal. LANA consists of a serine- and proline-rich N-terminal domain with a nuclear localization signal (NLS), a chromatin-binding motif (CBM), and domains responsible for interaction with the transcription regulators, mSin3 complex and Sp1. The central domain contains several highly repetitive acidic regions that vary in length and are responsible for the size variation of LANA proteins from different HHV-8 isolates. The proline-rich C-terminal third domain contains another NLS and is responsible for LANA dimerization and binding to the terminal repeats (TR) of the viral genomic DNA. HHV-8 infected latent cells are reported to induce the secretion of glutamate and activation of metabotropic glutamate receptor 1 (mGluR1). Elevated secretion of glutamate and mGluR1 activation has been linked to increased proliferation of HHV-8 infected cells. Use of glutamate release inhibitor and mGluR1 antagonists are shown to block the proliferation of infected cells. (Ref.: Kumar, B., et al. (2019). J. Virol. 93(9); e01812-18; Gjyshi, O., et al. (2015). J. Virol. 89(15); 7874-7892; Veettil, MV., et al. (2014). PLoS Pathog 10(10); e1004389).

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of Kaposi′s sarcoma-associated herpesvirus (HHV-8).

Quality Control Testing

Evaluated by Immunocytochemistry in TPA-induced BCBL-1 cell line.

Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected HHV-8 LANA1 in TPA-induced BCBL-1 cell line.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected HHV-8 LANA1 in Immunocytochemistry applications (Veettil, M.V., et al. (2014). PLoS Pathog. 10(10):e1004389).

Immunohistochemistry Applications: A representative lot detected HHV-8 LANA1 in Immunohistochemistry applications (Gjyshi, O., et al. (2015). J Virol. 89(15):7874-92).

Immunofluorescence Analysis: A representative lot detected HHV-8 LANA1 in Immunofluorescence applications (Gjyshi, O., et al. (2015). J Virol. 89(15):7874-92; Kumar, B., et al. (2019). J Virol.;93(9):e01812-18; Veettil, M.V., et al. (2014). PLoS Pathog. 10(10):e1004389).

Immunoprecipitation Analysis: A representative lot detected HHV-8 LANA1 in Immunoprecipitation applications (Gjyshi, O., et al. (2015). J Virol. 89(15):7874-92).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected HHV-8 LANA1 in Chromatin Immunoprecipitation applications (Gjyshi, O., et al. (2015). J Virol. 89(15):7874-92).

Western Blotting Analysis: A representative lot detected HHV-8 LANA1 in Western Blotting applications (Gjyshi, O., et al. (2015). J Virol. 89(15):7874-92; Veettil, M.V., et al. (2014). PLoS Pathog. 10(10):e1004389).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-HHV-8 LANA1, clone 1D10C3, Cat. No. MABF2759, is a mouse monoclonal antibody that detects LANA1 protein of HHV-8 and is used in ChIP, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Clone 1D10C3 is a mouse monoclonal antibody that detects LANA1 (ORF73) protein of HHV-8.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.