biological source
mouse
conjugate
PerCP-Cy5.5
antibody form
purified antibody
antibody product type
primary antibodies
clone
104, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
concentration
0.2 mg/mL
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Ptprc(19264)
General description
The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD45.2 (mouse), PerCP-Cy5.5, clone 104 validated for use in IP, Flow Cytometry & ICC for the detection of CD45.2.
Physical form
Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography
Preparation Note
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45.2 in one million C57Bl/6 splenocytes.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45.2 in one million C57Bl/6 splenocytes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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