Anti-GluR2 Antibody, clone L21/32

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this position is occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks Ca2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Recombinant protein corresponding to human GluR2.

Detect GluR2 using this Anti-GluR2 Antibody, clone L21/32 validated for use in WB, IH.

Immunohistochemistry Analysis: 1:300 dilution of this antibody from a previous lot detected GluR2 in rat cerebellum and hippocampus tissue.

Research Category
Neuroscience

Neuroscience

Research Sub Category
Neurodegenerative Diseases

Neurotransmitters & Receptors

Evaluated by Western Blot in rat brain membrane tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected GluR2 on 10 µg of rat brain membrane tissue lysate.

~ 96 kDa observed

Protein G

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat brain membrane tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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