Anti-Pirh2 Antibody, clone 11G8.1

Pirh2, also known as Androgen receptor N-terminal-interacting protein or CH-rich-interacting match with PLAG1 or E3 ubiquitin-protein ligase Pirh2 or RING finger protein 199 or Zinc finger protein 363 or p53-induced RING-H2 protein (Pirh2), encoded by the gene RCHY1/ARNIP/CHIMP/PIRH2/RNF199/ZNF363, is an E3 ubiquitin ligase protein that mediates the E3-dependent ubiquitination and proteasomal degradation of a number of important signaling and DNA methylation proteins including p53/TP53, p73, HDAC1 and CDKN1B. Pirh2 also monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. Additionally, Pirh2 increases androgen receptor transcription factor activity as well and Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, CDKN1B, COPE, UBE2D2 and GORAB/NTKLBP1 proteins. Pirh2 is localized to the cytoplasm and nucleus, and can be found in nucleus speckles. Pirh2 is expressed in all cell types examined and is up-regulated during the S phase of the cell cycle. Pirh2 is expressed at low levels during G phase.

GST-tagged recombinant protein corresponding to human Pirh2.

Anti-Pirh2 Antibody, clone 11G8.1 is a highly specific mouse monoclonal antibody & that targets Pirh2 & has been tested in western blotting.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Evaluated by Western Blotting in H1299 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Pirh2 in 10 µg of H1299 cell lysate.

~30 kDa observed

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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