Anti-DNA-PK (Ab-2) Mouse mAb (18-2)

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing the splenocytes with mouse myeloma SP2/0 cells (see application references). Recognizes the ~460 kDa DNA-PK protein.

Recognizes the ~470 kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.

This Anti-DNA-PK (Ab-2) Mouse mAb (18-2) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of DNA-PK (Ab-2).

DNA-PK purified from HeLa cells

Epitope: within amino acids 1-2713 of DNA-PK

Human

Frozen Sections (1:50-1:100)
Immunoblotting (1:250-1:500)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (10 l/mg protein lysate)
Neutralization Studies (see comments)
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)

Please refer to vial label for lot-specific concentration.

Toxicity: Standard Handling (A)

In 10 mM PBS, 0.2% BSA, pH 7.4.

For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Positive Control
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue

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This antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.

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