Anti-v-Src (Ab-1) Mouse mAb (327)

Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.

Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.

This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1).

Epitope: within the SH3 domain of v-Src and c-Src

purified v-Src

Immunoblotting (2.5 µg/ml, see application references)

Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Paraffin Sections (not recommended)

Please refer to vial label for lot-specific concentration.

Toxicity: Standard Handling (A)

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.

Positive Control
NIH/3T3 cells

Courtneidge, S.A. and Heber, A. 1987. Cell50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647.
Courtneidge, S.A. 1985. EMBO J.4, 1471.
Whitman, M., et al. 1985. Nature315, 239
Bolen, J.B., et al. 1984. Cell38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021.
Collett, M.S., et al. 1978. Cell15, 1363.

pp60^src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany