产品名称
抗层粘连蛋白抗体, Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity purified immunoglobulin
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
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相关类别
Immunogen
全长人层粘连蛋白。 通过SDS-PAGE评估单带纯度后发生免疫。
Application
使用经验证可用于 ELISA、IP、WB、IH的抗层粘连蛋白抗体检测层粘连蛋白。
免疫印迹: 1:500-1:2000
ELISA: 1:4000-1:8000
免疫组化: 1:50-1:200
免疫沉淀:条件必须由最终用户确定
最佳工作稀释度必须由最终用户进行确定。
ELISA: 1:4000-1:8000
免疫组化: 1:50-1:200
免疫沉淀:条件必须由最终用户确定
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
该产品是通过使用固定化抗原的免疫亲和层析制备的,然后针对人血清蛋白,胶原蛋白和非胶原细胞外基质蛋白进行广泛的交叉吸附,以去除不需要的反应性。 通常,通过ELISA针对纯化的标准品检测到与其他细胞外基质蛋白的交叉反应性低于1%。 该抗体与大多数哺乳动物层粘连蛋白反应,与I、II、III、IV、V或VI型胶原或纤维连接蛋白的交叉反应性可忽略不计。 抗层粘连蛋白抗体与其他人血清蛋白或非层粘连蛋白细胞外基质蛋白的非特异性交叉反应可忽略不计。
Physical form
亲和纯化的免疫球蛋白。 1 mg/mL液体,溶于0.125 M硼酸钠、0.075 M氯化钠、0.005 M EDTA(pH 8.0)和0.01%叠氮化钠中。
形式:纯化
Preparation Note
未稀释的抗体溶液在2-8° C下可稳定储存约6个月。对于延长储存期,与等体积甘油混合,等分并储存在-20°C下。 应避免反复冻/融循环。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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