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AB4124

Sigma-Aldrich

Anti-FOXA1 Antibody

Chemicon®, from rabbit

别名:

Forkhead Box Protein A1, Hepatocyte Nuclear Factor 3-alpha, HNF3A

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1 G
CN¥1,565.58
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CN¥5,368.86

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1 G
CN¥1,565.58
5 G
CN¥5,368.86

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥1,565.58


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生物来源

rabbit

质量水平

偶联物

unconjugated

抗体形式

affinity purified immunoglobulin

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

human, rat, mouse

制造商/商品名称

Chemicon®

技术

immunocytochemistry: suitable

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此商品
A6987C7118C6895
Cefdinir ≥97.0% (HPLC)

C7118

Cefdinir

Cefaclor

C6895

Cefaclor

mode of action

cell wall synthesis | interferes

mode of action

cell wall synthesis | interferes

mode of action

cell wall synthesis | interferes

mode of action

cell wall synthesis | interferes

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder or crystals

form

powder or crystals

form

powder or crystals

form

powder or crystals

assay

90.0-105.0%

assay

95.0-102.0% anhydrous basis

assay

≥97.0% (HPLC)

assay

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

特异性

Recognizes FOXA1. The calculated molecular weight is ~49.1 kDa.

免疫原

Synthetic peptide corresponding to amino acids 388-399 of human FOXA1 (LKGDPHYSFNHP).

应用

Anti-FOXA1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXA1 also known as Forkhead Box Protein A1, Hepatocyte Nuclear Factor 3-alpha, HNF3A & has been validated in ICC.
Immunocytochemistry: 1/200 to 1/1000.

Optimal dilutions must be determined by the user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

外形

Affinity purified immunoglobulin precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

储存及稳定性

Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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    1. How does the storage temperature relate to shipping conditions?

      The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

    2. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    3. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    4. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    5. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    6. What is the solution stability of the Product C3809, Ceftazidime hydrate?

      Unfortunately, we do not test Product C3809, Ceftazidime hydrate, for solution stability.  However, Chedru-Legros V. et al., Cornea, 29(7),807-11(2010), report 5% solutions of ceftazidime 0.9% sodium chloride can be stored at -20°C for 6 months. Also, Mehta, S. et al., Retina, 31(7),1316-22 (2011), reports single-use polypropylene syringes retain potency, sterility, and stability for 24 weeks when stored at -20°C or -80°C.

    7. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

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