AB5585
抗-恢复蛋白抗体
serum, Chemicon®
别名:
Recoverin
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, mouse, rat, frog, chicken
Application:
ICC, IHC (p), WB
Citations:
188
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat, frog, chicken
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... RCVRN(5957)
mouse ... Rcvrn(19674)
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General description
26kda
恢复蛋白是在视网膜感光细胞和微型锥体双极细胞中表达的钙结合蛋白。它似乎通过阻断光激发的视紫红质的磷酸化来延迟光转导级联的终止。
Immunogen
重组人恢复蛋白。
Application
使用经过验证可用于IC，IH，IH（P）& WB的该抗恢复蛋白抗体检测恢复蛋白。
研究子类别
感官 & PNS
感官 & PNS
研究类别
神经科学
神经科学
蛋白质印迹分析：
使用ECL为1：1,000-1：5,000稀释液。
免疫组化：
在冰冻和石蜡包埋的组织切片上为1：1,000（室温2小时或2-8ºC过夜）。建议的固定剂为4%多聚甲醛或3%多聚甲醛加0.1%戊二醛。建议的透化溶液是0.1%Triton X-100。建议的封闭缓冲液是0.05 M Tris盐水，pH值7.4，其中含有2.5%BSA，0.1%triton和0.05%叠氮化钠。
最佳工作稀释度必须由最终用户进行确定。
使用ECL为1：1,000-1：5,000稀释液。
免疫组化：
在冰冻和石蜡包埋的组织切片上为1：1,000（室温2小时或2-8ºC过夜）。建议的固定剂为4%多聚甲醛或3%多聚甲醛加0.1%戊二醛。建议的透化溶液是0.1%Triton X-100。建议的封闭缓冲液是0.05 M Tris盐水，pH值7.4，其中含有2.5%BSA，0.1%triton和0.05%叠氮化钠。
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
其他物种尚未经过测试。
可识别恢复蛋白。
Physical form
兔多克隆血清。不含防腐剂。
未纯化
Preparation Note
自收到之日起在-20ºC可稳定保存1年。
Analysis Note
对照
阳性对照：恢复蛋白存在于视网膜的光感受器和微型锥体双极细胞以及松果体的松果体细胞中。
阳性对照：恢复蛋白存在于视网膜的光感受器和微型锥体双极细胞以及松果体的松果体细胞中。
Other Notes
替代品：AB5431P
浓度：请参考批次特异性浓缩物的分析证书。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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