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Merck
CN

AB5803

抗-Sox4抗体

Chemicon®, from rabbit

别名:

Anti-Anti-CSS10, Anti-Anti-EVI16

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, WB
Citations:
11
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biological source

rabbit

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... SOX4(6659)

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Immunogen

合成肽,Sox4的34-50位氨基酸。

Application

抗-Sox4抗体是一种高质量的兔多克隆抗体,用于检测Sox4,&已在WB,ICC中验证。

Biochem/physiol Actions

可识别Sox4。蛋白质的分子量为47,258道尔顿。

Preparation Note

将未开封的小瓶在-70°C保存6个月应避免反复冻/融循环。

制备和使用:

为了复溶抗体,以中等速度(~10,000G)离心抗体小瓶15分钟,以颗粒化沉淀的抗体产物。底部附近会有白色颗粒。小心取出硫酸铵/PBS缓冲溶液并丢弃。该过程不必一定要移出所有的硫酸铵/PBS溶液。10 μL残留硫酸铵溶液不会影响抗体的重悬。不要使蛋白颗粒干燥,因为可能会导致抗体反应性严重丧失。

用合适的生物缓冲液如PBS或TBS(pH值7.3-7.5)重悬抗体颗粒,使其最终浓度为1.0 mg/mL。例如,为了用50 μg沉淀的抗体达到1.0 mg/mL的浓度,所需的缓冲液量为50μL。

小心地将液体缓冲液添加到颗粒上。不可涡旋。用宽移液器吸头轻轻搅拌或用手指轻敲混匀。使用前,将沉淀的抗体在4-25°C下再水化1小时。无法重悬的沉淀的抗体小颗粒是正常的。过量灌装小瓶以补偿任何损失。

再水化的抗体溶液可以在2-8°C下以未稀释的形式储存2个月,而没有任何明显的活性损失。注意,该溶液不是无菌的,因此如果产品在2-8°C下储存,则应格外小心。

对于在-20°C下储存,可以加入等体积的甘油,但是,建议使用ACS级或更高等级的甘油,因为如果使用的甘油不是高质量,则可能会导致活性显著损失。

对于在-70°C下的长期储存,建议使用再水化缓冲液制备的2%BSA(组分V,可用的最高等级)溶液进一步按1:1稀释再水化抗体溶液。可以将得到的1%BSA/抗体溶液等分,并在-70°C下冷冻保存长达6个月。应避免反复冻/融循环。

Other Notes

替代品:AB9092

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2


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