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Merck
CN

AB733

Sigma-Aldrich

Anti-Tamm-Horsfall Glycoprotein Antibody

serum, Chemicon®

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源

sheep

抗体形式

serum

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

human

制造商/商品名称

Chemicon®

技术

immunodiffusion: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... UMOD(7369)

免疫原

Highly purified human Tamm-Horsfall glycoprotein (uromucoid) from urine; >95% pure by SDS-PAGE.

应用

IEP: 100 μL antiserum vs. 5 μL uromucoid (50 mg/L)

RID and Rocket RID: 10 μL antiserum/cm2 in gel vs. 5 μL uromucoid (50 mg/L), NT - 1/5

Double diffusion: 10 μL antiserum vs. 3 μL uromucoid (50 mg/L)

*Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended for the above applications.

Immunohistochemistry

Optimal working dilution must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-Tamm-Horsfall Glycoprotein Antibody is validated for use in ID, WB, IH for the detection of Tamm-Horsfall Glycoprotein.

生化/生理作用

One arc by 2D-IEP against Tamm-Horsfall glycoprotein (1 mg/mL). No precipitin line by IEP against normal human plasma. Identity verified by double immunodiffusion.

外形

In glycine buffered saline, pH 7.4 containing 0.1% sodium azide, 0.1% E-Amino-n-Caproic Acid, 0.01% Benzamidine and 1 mM Ethylenediaminetetraacetic acid as preservatives.

制备说明

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Brian M Nolen et al.
PloS one, 8(5), e63368-e63368 (2013-06-01)
The analysis of protein biomarkers in urine is expected to lead to advances in a variety of clinical settings. Several characteristics of urine including a low-protein matrix, ease of testing and a demonstrated proteomic stability offer distinct advantages over current
Jenni Karttunen et al.
International journal of molecular sciences, 22(9) (2021-06-03)
Urinary extracellular vesicles (EVs) and their RNA cargo are a novel source of biomarkers for various diseases. We aimed to identify the optimal method for isolating small (<200 nm) EVs from human urine prior to small RNA analysis. EVs from
Jean-Claude Kresse et al.
Acta physiologica (Oxford, England), 234(3), e13780-e13780 (2022-01-07)
Renal fibrosis is a major driver of chronic kidney disease, yet current treatment strategies are ineffective in attenuating fibrogenesis. The cyclooxygenase/prostaglandin system plays a key role in renal injury and holds great promise as a therapeutic target. Here, we used
Somchai Chutipongtanate et al.
The Journal of clinical investigation, 115(12), 3613-3622 (2005-11-26)
Previous research on proteins that inhibit kidney stone formation has identified a relatively small number of well-characterized inhibitors. Identification of additional stone inhibitors would increase understanding of the pathogenesis and pathophysiology of nephrolithiasis. We have combined conventional biochemical methods with
Fernanda Chacar et al.
Physiological reports, 5(11) (2017-06-04)
Proteinuria is a marker and mediator of chronic kidney disease (CKD). In clinical practice, the urinary protein-to-creatinine ratio (UP/C) is of limited usefulness, because it indicates only the magnitude of proteinuria and not the origin of the loss (glomerular or

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