生物来源
rabbit
抗体形式
serum
克隆
polyclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
免疫原
The antigen used in the production of this antiserum is serotonin covalently bound to bovine thyroglobulin with carbodiimide.
应用
Detect Serotonin using this Anti-Serotonin Antibody validated for use in IF, IH(P).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
TISSUE EVALUATION:
Formalin-fixed and paraffin embedded sections (5-6 microns thick) of human small intestine were used in the evaluation of this antiserum.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:1,000-1:2,000 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3′ -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
Formalin-fixed and paraffin embedded sections (5-6 microns thick) of human small intestine were used in the evaluation of this antiserum.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:1,000-1:2,000 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3′ -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
生化/生理作用
The antiserum provided has been evaluated for its ability to stain serotonin-containing structures in the ileum of man. Staining is positive using both the immunoperoxidase or immunofluorescence procedures.
外形
Liquid antiserum. Contains 0.1% sodium azide.
制备说明
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
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