biological source
rabbit
antibody form
serum
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable, immunohistochemistry: suitable (paraffin)
Immunogen
The antigen used in the production of this antiserum is serotonin covalently bound to bovine thyroglobulin with carbodiimide.
Application
Detect Serotonin using this Anti-Serotonin Antibody validated for use in IF, IH(P).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
TISSUE EVALUATION:
Formalin-fixed and paraffin embedded sections (5-6 microns thick) of human small intestine were used in the evaluation of this antiserum.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:1,000-1:2,000 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3′ -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
Formalin-fixed and paraffin embedded sections (5-6 microns thick) of human small intestine were used in the evaluation of this antiserum.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:1,000-1:2,000 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3′ -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
Biochem/physiol Actions
The antiserum provided has been evaluated for its ability to stain serotonin-containing structures in the ileum of man. Staining is positive using both the immunoperoxidase or immunofluorescence procedures.
Physical form
Liquid antiserum. Contains 0.1% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
存储类别
10-13 - German Storage Class 10 to 13
法规信息
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