生物来源
rabbit
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse, human
技术
ChIP: suitable (ChIP-seq)
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
acetylation (Lys5,Lys7,Lys11)
基因信息
human ... H2AFZ(3015)
一般描述
H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
~16 kDa observed
免疫原
KLH-conjugated linear peptide corresponding to human acetyl Histone H2A.Z (Lys 5, 7, 11) near the N-terminus.
应用
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from HeLa cells.
Dot Blot Analysis: 0.05 ug/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in H2A.Z peptides.
Chromatin Immunoprecipitation (Seq.) Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from K562 cells.
Immunocytochemistry Analysis: A representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in HeLa cells.
Dot Blot Analysis: 0.05 ug/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in H2A.Z peptides.
Chromatin Immunoprecipitation (Seq.) Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from K562 cells.
Immunocytochemistry Analysis: A representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in HeLa cells.
This Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody is validated for use in Western Blotting and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Dot Blot and ELISA for the detection of acetyl Histone H2A.Z (Lys 5, 7, 11).
生化/生理作用
Human, mouse, wide range expected.
外形
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 0.05% ProClin300.
分析说明
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in 10 µg of sodium butyrate treated HeLa acid extract.
其他说明
Concentration: Please refer to lot specific datasheet.
未找到合适的产品？
试试我们的产品选型工具.
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Anas Reda et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 49(8), 1285-1295 (2024-02-17)
Creating long-lasting memories requires learning-induced changes in gene expression, which are impacted by epigenetic modifications of DNA and associated histone proteins. Post-translational modifications (PTMs) of histones are key regulators of transcription, with different PTMs producing unique effects on gene activity
Carissa J Dunn et al.
eNeuro, 4(4) (2017-09-01)
The histone variant H2A.Z is an essential and conserved regulator of eukaryotic gene transcription. However, the exact role of this histone in the transcriptional process remains perplexing. In vertebrates, H2A.Z has two hypervariants, H2A.Z.1 and H2A.Z.2, that have almost identical
Tara Procida et al.
International journal of molecular sciences, 22(2) (2021-01-16)
Histone variants differ in amino acid sequence, expression timing and genomic localization sites from canonical histones and convey unique functions to eukaryotic cells. Their tightly controlled spatial and temporal deposition into specific chromatin regions is accomplished by dedicated chaperone and/or
BreAnna Cameron et al.
Biology open, 10(9) (2021-10-01)
Elongator dysfunction is increasingly recognized as a contributor to multiple neurodevelopmental and neurodegenerative disorders including familial dysautonomia, intellectual disability, amyotrophic lateral sclerosis, and autism spectrum disorder. Although numerous cellular processes are perturbed in the context of Elongator loss, converging evidence
Klotilda Narkaj et al.
eNeuro, 5(5) (2018-11-13)
Memory formation is a protracted process that initially involves the hippocampus and becomes increasingly dependent on the cortex over time, but the mechanisms of this transfer are unclear. We recently showed that hippocampal depletion of the histone variant H2A.Z enhances
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持